平台授权一级代理,咨询微信:jinshanbio
Bsp143I (Sau3AI) (10 U/µL)品牌:Fermentas | 货号:ER0782
Enzyme | Bsp143I (Sau3AI) |
---|
保存温度 | -20℃ |
---|
货期 | 2-3天 |
---|
Compatible Buffer | Unique Buffer (10x Buffer Bsp143I (Sau3AI)) |
---|
Optimal Reaction Temperature | 37° C |
---|
Sensitive to Heat Inactivation: | Yes |
---|
Methylation Sensitivity | CpG methylation-sensitive,Not dam methylation-sensitive,Not dcm methylation-sensitive |
---|
Thermo Scientific Bsp143I (Sau3AI) restriction enzyme recognizes ^GATC sites and cuts best at 37°C in its own unique buffer (Isoschizomers: BfuCI, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, NdeII, Sau3AIm). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Features
• Superior quality—stringent quality control and industry leading manufacturing process• Convenient color-coded Five Buffer System• Includes universal Tango buffer for double-digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism (RFLP)• SNP
Note: DpnI, Bsp143I, and MboI all recognize the same sequence, but have different methylation sensitivities and cleavage sitesFor methylation sensitivity, refer to product specifications.
授权一级代理请认准金山科研平台,产品价格和货期请咨询微信:jinshanbio
,询价请加微信:jinshanbio
©
金山科研平台 Bio-Rad伯乐官网 |伯乐授权代理 |伯乐ddPCR| 伯乐Aminex| 伯乐层析填料色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价,请加客服QQ:1749072012 、客服微信:jinshanbio,或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务,数据资源基于CC协议。
© 本文地址:
https://brandmillipore.cn/thread-59645.htm