INVITROGEN反转录酶II，SuperScript II Reverse Transcriptase货号18061014

描述

反转录（RT）superscript®II是一个改进版本superscript®RT。It is a聚合酶，DNA链synthesizes有互补单链RNA从DNA、RNA、DNA杂交。这种酶是转基因工程by the introduction of点突变in the RNase H活性中心to reduce RNase H活性。这eliminates降解RNA分子结构的变化在*链cDNA Synthesis and gives superscript®II RT性能特性上。

INVITROGEN反转录酶II，SuperScript II Reverse Transcriptase货号18061014

SuperScript® II Reverse Transcriptase (RT) is an improved version of SuperScript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript® II RT superior performance characteristics.

•Yield– Reduced RNase H activity results in greater first-strand cDNA yields

•Size of cDNA– Generation of RT-PCR products up to 12 kb

•Thermostability– Full activity at 42°C

•Flexibility– Uses either total or poly(A)+RNA

Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product.

Unit Definition: One unit of SuperScript® II is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)•oligo(dT)12-18as template•primer (3).

Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18, 0.1 mg/ml BSA, and enzyme in 50 µl for 10 min. at 37°C.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

ase货号18061014

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