STEMCELL淋巴细胞分离液Lymphoprep
*替代Ficoll-Paque
工作原理
PBMC与血液中的其他成分存在密度差异，利用密度在1.077±0.001g/ml之间而且近于等渗的LymphoprepTM溶液作密度梯度离心时，红细胞和粒细胞由于密度大，故沉于分离液底部。PBMC密度稍低于分离液，故位于分离液界面上，获得PBMC。LymphoprepTM分离液含有多糖（polysacccharide）,多糖能粘附在细胞表面，使红细胞和粒细胞聚集下沉，但它对淋巴细胞的活性和红细胞和粒细胞进一步分离有负面影响。
STEMCELL淋巴细胞分离液Lymphoprep
注意事项：
1、该分离液仅适用于人体的外周血液，不适用于动物。
2、使用该分离液时，需将待分离的血液用生理盐水做等体积的稀释。
3、分离液的保存应置于无菌环境，并避光保存。
4、详细操作步骤见产品说明书。
技术参数
成分：泛影葡胺钠(Sodium diatrizoate)：9.1% (w/v)；多糖：5.7% (w/v)
密度：1.077±0.001g/ml
渗透性：290±15mOsm
内毒素含量：<1.0EU/ml
分离所得PBMC中红细胞的含量：整个细胞数目的3-10%。
Lymphoprep™ is a density gradient medium recommended for the isolation of mononuclear cells from peripheral blood, cord blood, and bone marrow by exploiting differences in cell density. Granulocytes and erythrocytes have a higher density than mononuclear cells and therefore sediment through the Lymphoprep™ layer during centrifugation. Lymphoprep can be substituted for Ficoll-Paque™ without any need to change your existing protocols and is fully compatible with both Sepmate™ and RosetteSep™.
| Product Type: | Cell isolation products |
| Recommended For: | Isolation of mononuclear cells from peripheral blood, cord blood, and bone marrow |
| Components: | • Sodium Diatrizoate 9.1% (w/v) • Polysaccharide 5.7% (w/v) • Water • Density of 1.077 g/mL |
| Accessory Products: | SepMate™-50 (Catalog #15450/15460) |
| Contains: | • Sodium Diatrizoate (9.1% w/v) • Polysaccharide (5.7% w/v) • Density of 1.077 g/mL |
| Equipment Required: | Centrifuge |
| Cell Isolation Product Type: | Reagents |
| Cell Source: | Bone marrow, Cord blood, Whole blood |
| Legal Statement: | Lymphoprep™ is a trademark of Axis-Shield. |
| Intended Use Statement: | FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES. |
| ISO Statement: | STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS. |
订购信息：
07851 500ml
07861 6*500ml
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