VECTOR免疫组化试剂盒VECTASTAIN ABC Kit(PK-6100)
Vector Laboratories公司是世界著名的生物学检测试剂公司。1977年首先开发出了生物素-亲和素系统,该产品系列是检测方法上的重要革命。之后经过不懈的努力,VectorLaboratories公司发展出ABC技术(Avidin : Biotinylated Enzyme ComplexTechnology,亲和素-生物素-过氧化物酶复合物技术)并建立了著名的VECTASTAIN@ ABC系统,该系统被普遍视为目前zui灵敏、zui可靠与zui有效的染色系统,并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。而该系统仍在不断地发展,以满足广大研究人员的各种不同要求(如多抗原的标记检测)
VECTOR免疫组化试剂盒VECTASTAIN ABC Kit(PK-6100)
ABC技术利用生物素和卵白素具有*亲和性的生物学特征,将卵白素和生物素化辣根酶按照一定的比例混合,形成ABC复合物。该法亦被称作三步法,*步为biotin化二抗和一抗结合,第二步为avidin(亲和素,A液)和二抗上的biotin结合,第三步为HRP偶联的biotin(B液)和avidin结合,而HRP再催化底物显色完成染色。
VECTOR免疫组化试剂盒VECTASTAIN ABC Kit(PK-6100)
Features:Advanced avidin/biotin technology: The Elite
®ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated targetHIghest sensitivity, low background: The VECTASTAIN
®Elite
®ABC system is the most sensitive avidin/biotin-based peroxidase system. The Elite
®ABC series is approximay 5 times more sensitive than the original VECTASTAIN
®ABC Kit with the same low background.Cost effective: HIgher sensitivity leads to lower cost per slideAvailable without ("Standard" kit) or with biotinylated species-specific or universal secondary antibodiesAvailable in ready-to-use formats: Prediluted, stabilized working solutions of Elite
®ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN
®Elite
®ABC Kit reagents.
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