SANTA CRUZ BIOTECHNOLOGY, INC.

Talin-1 （YQ-16）: sc-81805

Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com

BACKGROUND

Focal adhesions were identified as areas within the plasma membrane of

tissue culture cells that adhere tightly to the underlying substrate. In vivo,

these regions are involved in the adhesion of cells to the extracellular matrix.

Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components

of a protein complex that links the Actin network to the plasma

membrane. Vinculin binding sites have been identified on other cytoskeletal

proteins, including Talin-1 and α-actinin. In addition, vinculin, Talin-1, Talin-2

and α-actinin each contain Actin binding sites. Expression of vinculin, Talin-1

and Talin-2 have been shown to be affected by the level of Actin expression.

α-actinin has been shown to link Actin to integrins in the plasma membrane

through interactions with the vinculin and Talin complex or by a direct interaction

with integrin. Talin-2 is similar to Talin-1 but shows distinct patterns of

expression and cannot compensate for the loss of Talin-1.

REFERENCES

1. Burridge, K., et al. 1988. Focal adhesions: transmembrane junctions

between the extracellular matrix and the cytoskeleton. Annu. Rev. Cell

Biol. 4: 487-525.

2. Gilmore, A.P., et al. 1992. Further characterization of the Talin-binding site

in the cytoskeletal protein vinculin. J. Cell Sci. 103: 719-731.

3.Wood, C.K., et al. 1994. Characterisation of the paxillin-binding site and

the C-terminal focal adhesion targeting sequence in vinculin. J. Cell Sci.

107: 709-717.

4. Gluck, U., et al. 1994. Modulation of α-actinin levels affects cell motility

and confers tumorigenicity on 3T3 cells. J. Cell Sci. 107: 1773-1782.

5. Schevzov, G., et al. 1995. Impact of Actin gene expression on vinculin,

Talin, cell spreading, and motility. DNA Cell Biol. 14: 689-700.

6. Hemmings, L., et al. 1996. Talin contains three Actin-binding sites each of

which is adjacent to a vinculin-binding site. J. Cell Sci. 109: 2715-2726.

7. Gilmore, A.P., et al. 1996. Regulation of vinculin binding to Talin and Actin

by phosphatidyl-inositol-4-5-bisphosphate. Nature 381: 531-535.

CHROMOSOMAL LOCATION

Genetic locus: TLN1 （human） mapping to 9p13.3; Tln1 （mouse） mapping to

4 B1.

SOURCE

Talin-1 （YQ-16） is a mouse monoclonal antibody raised against recombinant

Talin-1 of human origin.

PRODUCT

Each vial contains 100 μg IgG3 in 1.0 ml PBS with < 0.1% sodium azide and

0.1% gelatin.

STORAGE

Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of

shipment. Non-hazardous. No MSDS required.

APPLICATIONS

Talin-1 （YQ-16） is recommended for detection of Talin-1 of mouse, rat and

human origin by Western Blotting （starting dilution 1:200, dilution range

1:100-1:1000）, immunoprecipitation [1-2 μg per 100-500 μg of total protein

（1 ml of cell lysate）], immunofluorescence （starting dilution 1:50, dilution

range 1:50-1:500）, immunohistochemistry （including paraffin-embedded

sections） （starting dilution 1:50, dilution range 1:50-1:500） and solid phase

ELISA （starting dilution 1:30, dilution range 1:30-1:3000）.

Suitable for use as control antibody for Talin-1 siRNA （h）: sc-36610, Talin-1

siRNA （m）: sc-36611, Talin-1 shRNA Plasmid （h）: sc-36610-SH, Talin-1 shRNA

Plasmid （m）: sc-36611-SH, Talin-1 shRNA （h） Lentiviral Particles: sc-36610-V

and Talin-1 shRNA （m） Lentiviral Particles: sc-36611-V.

Molecular Weight of Talin-1: 230 kDa.

Positive Controls: RAW 264.7 whole cell lysate: sc-2211.

RECOMMENDED SECONDARY REAGENTS

To ensure optimal results, the following support （secondary） reagents are

recommended: 1） Western Blotting: use goat anti-mouse IgG-HRP: sc-2005

（dilution range: 1:2000-1:32,000） or Cruz Marker™ compatible goat antimouse

IgG-HRP: sc-2031 （dilution range: 1:2000-1:5000）, Cruz Marker™

Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent:

sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2） Immunoprecipitation:

use Protein A/G PLUS-Agarose: sc-2003 （0.5 ml agarose/2.0 ml）.

3） Immunofluorescence: use goat anti-mouse IgG-FITC: sc-2010 （dilution

range: 1:100-1:400） or goat anti-mouse IgG-TR: sc-2781 （dilution range:

1:100-1:400） with UltraCruz™ Mounting Medium: sc-24941. 4） Immunohistochemistry:

use ImmunoCruz™: sc-2050 or ABC: sc-2017 mouse IgG

Staining Systems.

DATA

SELECT PRODUCT CITATIONS

1. Ah Kioon, M.D., et al. 2010. Adrenomedullin increases fibroblast-like synoviocyte

adhesion to extracellular matrix proteins by upregulating integrin

activation. Arthritis Res. Ther. 12: R190.

RESEARCH USE

For research use only, not for use in diagnostic procedures.

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