Pharmcia（GE)葡聚糖凝胶G-75

Sephadex G-75, 100 g

Sephadex G-75 is well established gel filtration medium for desalting and buffer exchange of large biomolecules >80 000 molecular weight.

Quickly desalts, removes contaminants and transfers to a new buffer in a single step.Suitable for buffer exchange and cleanup of large biomolecules, >80 000 molecular weightHighest exclusion limit, 80 000, in the Sephadex G-rangeClassic gel filtration medium.

Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-10 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.

Pharmcia（GE)葡聚糖凝胶G-75

Sephadex G-75, 100 g

Matrix	Cross-linked dextran
Particle Size Wet	90 µm-280 µm
Partical Size Dry	40 µm-120 µm
pH Stability Working Range	2-10
Flow Rate	< Darcy´s Law1)
Pressure/Flow Specification	min 90 cm/h, bed height 10 cm, column 5 cm i.d.
Fractionation [Mr] Globular Proteins	3 x 103 – 8 x 104
Fractionation [Mr] Dextrans	1 x 103-5 x 104
Exclusion Limit	> 7 x 104
Storage Conditions	4 to 30°C
Autoclavable	In wet form (pH 7.0) at 120°C for 30 min
1)The beads behave as rigid spheres obeying Darcy's Law.

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