RD Recombinant human IL-17A人白介17因子

DESCRIPTION

Source E. coliderived

Thr22Ala158

Accession # Q62386.1

Nterminal

Sequence

Analysis

Thr22

Structure / Form Disulfidelinked

homodimer

Predicted Molecular

Mass

15.5 kDa （monomer）

SPECIFICATIONS

Activity Measured by its ability to induce IL6

secretion by NIH-3T3 mouse embryonic fibroblast cells. Yao, Z. et al. （1995） Immunity 3:811.

The ED50 for this effect is typically 0.251.25

ng/mL.

Endotoxin Level <1.0 EU per 1 μg of the protein by the LAL method.

Purity >97%, by SDSPAGE

under reducing conditions and visualized by silver stain.

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. See Certificate of Analysis for details.

PREPARATION AND STORAGE

Reconstitution Reconstitute at 25 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Shipping The product is shipped at ambient temperature. Upon receipt, store it immediay at the temperature recommended below.

Stability & Storage Use a manual defrost freezer and avoid repeated freezethaw

cycles.

l 12 months from date of receipt, 20

to 70

°C as supplied.

l 1 month, 2 to 8 °C under sterile conditions after reconstitution.

l 3 months, 20

to 70

°C under sterile conditions after reconstitution.

BACKGROUND

Interleukin 17 （also known as CTLA8）

is a T cellexpressed

pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of

herpesvirus Saimiri. cDNA clones encoding IL17

have been isolated from activated rat, mouse and human T cells. Mouse IL17

cDNA encodes a 158 amino acid （aa）

residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield the 137 aa residue mature IL17.

Both recombinant and natural IL17

have

been shown to exist as disulfide linked homodimers. At the amino acid level, mIL17

shows 57% and 87% sequence identity with herpesvirus and rat IL17,

respectively. An IL17

specific mouse cell surface receptor （IL17

R） has been cloned. While the expression of IL17

mRNA is restricted to activated alpha beta

TCR+CD4CD8T

cells, the expression of mIL17

R mRNA has been detected in virtually all cells and tissues tested. IL17

exhibits multiple biological activities on a

variety of cells including: the induction of IL6

and IL8

production in fibroblasts? the enhancement of surface expression of ICAM1

in fibroblasts? activation of NFκB

and costimulation of T cell proliferation.

References:

1. Kennedy, J. et al., （1996） J. Interferon Cytokine Res. 16:611.

2. Yao, Z. et al., （1995） J. Immunol. 155:5483.

3. Yao, Z. et al., （1995） Immunity 3:811.

4. Rouvier, E. et al., （1993） J. Immunol. 150:5445.

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