Anti-HE4 antibody
Description
Rabbit polyclonal to HE4
Tested applications
WB, IHC-Pmore details
Cross reactivity
Anti-HE4 antibody
Reacts with
Human
Immunogen
Human mature HE4 fused to glutathione S-transferase. Produced in E.coli.
Positive control
Normal human epididymis or human ovarian tumour.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, BSA
Concentration
100 µl at 0.03mg/ml
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Datasheet PDFSDS
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HE4 antibody (ab24480)(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab24480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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WB
WB: 1/100. Detects a ban...Read more →
WB: 1/100. Detects a band of approximay 25 kDa (predicted molecular weight: 13 kDa).
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IHC-P
IHC-P: 1/40. Perform hea...Read more →
IHC-P: 1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Target
Tissue specificity
Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis.
Sequence similarities
Contains 2 WAP domains.
Cellular localization
Secreted.
Target information above from: UniProt accessionQ14508The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Information by UniProt
Form
There are 5 isoforms produced by alternative splicing.
Alternative names
dJ461P17.6 antibodyEDDM4 antibodyepididymal protein 4 antibodyEpididymal secretory protein E4 antibodyEpididymis specific whey acidic protein type four disulfide core antibodyHE 4 antibodyMajor epididymis specific protein E4 antibodyMajor epididymis-specific protein E4 antibodyMGC57529 antibodyPutative protease inhibitor WAP5 antibodyWAP 5 antibodyWAP domain containing protein HE4 antibodyWAP four disulfide core domain 2 antibodyWAP four disulfide core domain protein 2 antibodyWAP four-disulfide core domain protein 2 antibodyWAP5 antibodyWFDC 2 antibodyWFDC2 antibodyWFDC2_HUMAN antibody
see all
Database links
Entrez Gene: 10406 HumanSwissProt: Q14508 HumanUnigene: 2719 Human
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