Fermentas-Nt.Bpu10I(5U/µL)-限制性内切酶-分子生物学

2023-10-29

平台授权一级代理,咨询微信:jinshanbio

Nt.Bpu10I (5 U/µL)品牌:Fermentas | 货号:ER2011

EnzymeNt.Bpu10I
保存温度-20℃
货期2-3天
Compatible Buffer10x Buffer R
Optimal Reaction Temperature37° C
Sensitive to Heat Inactivation:Yes

5′CC↓TNAGC3′
3′GGANTCG5′

Thermo Scientific Nt.Bpu10I nicking enzyme recognizes CC^TNAGC sites and nicks best at 37°C in R buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—stringent quality control and industry leading manufacturing process• Convenient color-coded Five Buffer System• Includes universal Tango buffer for double-digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificities

ApplicationsProduction of single-stranded circular DNA from supercoiled double-stranded plasmids in vitro with subsequent use in DNA sequencing, site-specific mutagenesis, etc Creation of nested deletions Vector preparation for ligation independent cloning method Preparations of covalently closed, double- stranded linear DNA molecules

Note: Nt.Bpu10I may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid atypical DNA band patterns, use the 6X DNA Loading Dye & SDS Solution for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis.

授权一级代理请认准金山科研平台,产品价格和货期请咨询微信:jinshanbio ,询价请加微信:jinshanbio

© 金山科研平台 Bio-Rad伯乐官网 |伯乐授权代理 |伯乐ddPCR| 伯乐Aminex| 伯乐层析填料色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价,请加客服QQ:1749072012 、客服微信:jinshanbio,或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务,数据资源基于CC协议。
© 本文地址:https://brandmillipore.cn/thread-59956.htm
产品询价需求提交
返回
扫码添加客服微信,咨询产品报价