品牌: wshtbio 公司: 上海万生昊天生物技术有限公司

货号： SWSPA002 抗体英文名： GAPDH polyclonal antibody 供应商： 上海万生昊天生物技术有限公司 克隆性： 否 规格： 100ul

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BackgroundGlyceraldehyde-3-phosphate dehydrogenase (GAPDH), also called uracil DNA glycosylase, catalyzes the reversible oxidative phosphorylation of glyceraldehyde- 3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide, an important energy yielding step in carbohydrate metabolism. GAPDH is a 37KDa glycolytic enzyme constitutively and stably expressed in almost all tissues.Proteins such as GAPDH involved in maintenance of basic cellular function are often referred to as housekeeping protein that are frequently used as loading controls for western blots and protein normalization.DescriptionA highly specific and sensitive rabbit polyclonal antibody against GAPDH.SourceKLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GAPDH.ApplicationsWB 1:1000-2000, ICH 1:500-2000. Optimal dilution has to be determined by the user.Applicable Speciesmouse, rat, human.SpecificationsEach vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4, 0.5% BSA with 0.05% sodium azide. Antibody was purified by affinity chromatography.Storage conditionsstore at 2-8℃ for 3 months, -20℃ for 1 year. To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20℃) or for long-term storage (-80℃).NoteFor research use only, not for use in diagnostic procedures.DataWestern blot analysis of GAPDH expression in Rat liver (1), HeLa (2), Sp/20 (3) and NIH/3T3 (4) whole cell lysates