THERMO T4 DNA连接酶,T4 DNA Ligase

Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.

T4 DNA Ligase requiresATPas a cofactor.

Highlights

• Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP)

• Fast—sticky-end ligation is completed in 10 minutes at room temperature

• Supplied with PEG solution for efficient blunt-end ligation

THERMO T4 DNA连接酶,T4 DNA Ligase

订购信息：

THERMO T4 DNA连接酶,T4 DNA Ligase

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