Activity Measured by its ability to induce IL6

secretion by NHDF human normal dermal fibroblasts.

The ED50 for this effect is typically 26

ng/mL.

Measured by its ability to induce IL6

secretion by NIH-3T3 mouse embryonic fibroblast cells. Yao, Z. et al. （1995） Immunity 3:811.

The ED50 for this effect is typically 1.57.5

ng/mL.

Endotoxin Level <1.0 EU per 1 μg of the protein by the LAL method.

Purity >97%, by SDSPAGE

under reducing conditions and visualized by silver stain.

Formulation Lyophilized from a 0.2 μm filtered solution in HCl. See Certificate of Analysis for details.

PREPARATION AND STORAGE

Reconstitution Reconstitute at 100 μg/mL in 4 mM HCI.

Shipping The product is shipped with polar packs. Upon receipt, store it immediay at the temperature recommended below.

Stability & Storage Use a manual defrost freezer and avoid repeated freezethaw

cycles.

l 12 months from date of receipt, 20

to 70

°C as supplied.

l 1 month, 2 to 8 °C under sterile conditions after reconstitution.

l 3 months, 20

to 70

°C under sterile conditions after reconstitution.

BACKGROUND

Interleukin 17 （also known as CTLA8）

is a T cellexpressed

pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of

herpesvirus Saimiri. cDNA clones encoding IL17

have been isolated from activated rat, mouse and human T cells. Human IL17

cDNA encodes a 155 amino acid （aa）

residue precursor protein with a 19 amino acid residue signal peptide that is cleaved to yield the 136 aa residue mature IL17

containing one potential Nlinked

glycosylation site. Both recombinant and natural IL17

have been shown to exist as disulfidelinked

homodimers. At the amino acid level, human IL17

shows 72% and

63% sequence identity with herpesvirus and rat IL17,

respectively. An IL17

specific mouse cell surface receptor （IL17

R） has been cloned. While the expression of

IL17

mRNA is restricted to activated T cells, the expression of mIL17

R mRNA has been detected in virtually all cells and tissues tested. IL17

exhibits multiple

biological activities on a variety of cells including the induction of IL6

and IL8

production in fibroblasts, the enhancement of surface expression of ICAM1

in

fibroblasts, activation of NFκB

and costimulation of T cell proliferation. The nonglycosylated,

E. coliexpressed

rhIL17

has been shown to be active on human as

well as mouse cell lines.

References:

1. Yao, Z. et al. （1995） J. Immunol. 155:5483.

2. Yao, Z. et al. （1995） Immunity 3:811.

3. Rouvier, E. et al. （1993） J. Immunol. 150:5445

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