抗泛素抗体Anti-Ubiquitin antibody [Ubi-1]

Description Mouse monoclonal [Ubi-1] to Ubiquitin

Host species Mouse

Specificity Ubi-1 was initially screened on ELISA of the immunogen, and subsequently tested on sections of Alzheimer brain. It was one of several

clones which stained neurofibrillary tangles in frozen sectioned material strongly and specifically. Subsequent studies indicated that Ubi-

1 is relatively insensitive to formalin fixation and so can be used on mildly fixed histological sections of human brain for studies of

Alzheimer's disease. Ubi-1 also recognizes other ubiquitinated inclusion bodies such as the Lewy bodies of Parkinson's disease and

the Pick bodies in Pick's disease. This antibody recognizes polyubiquitin chains much more strongly than monoubiquitinated molecules

or free ubiquitin. Specifically recognizes ubiquitinated cytoplasmic inclusion bodies.

Tested applications IHC-P, WB, ELISA, ICC/IF, IHC-Fr

Cross reactivity Reacts with

Mouse, Chicken, Cow, Human, Arabidopsis thaliana, Caenorhabditis elegans, Fruit fly （Drosophila melanogaster）

Immunogen Full length native protein （purified） conjugated to KLH by a Glutaraldehyde linker.

Target

Relevance Ubiquitin is a highly conserved protein of about 8.5 kDa molecular weight, which has an ATP dependent role in the targeting of proteins

for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin,

and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently

bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies

are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of

Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated

and can be readily visualized with ubiquitin antibodies.

Cellular localization Cell Membrane, Cytoplasmic and Nuclear

Alternative names FLJ25987 antibody

MGC8385 antibody

Polyubiquitin B antibody

RPS 27A antibody

RPS27A antibody

UBA 52 antibody

UBA 80 antibody

UBA52 antibody

UBA80 antibody

UBB antibody

UBC antibody

UBCEP 1 antibody

UBCEP 2 antibody

UBCEP1 antibody

UBCEP2 antibody

Ubiquitin antibody

ubiquitin B antibody

Properties

Form Liquid

Storage instructions Store at +4°C short term （1-2 weeks）. Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

抗泛素抗体Anti-Ubiquitin antibody [Ubi-1]反应性有其他种属的：人，小鼠，大鼠等等

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