santacruz PCGF6 （E-15） 抗体*

BACKGROUND

Polycomb group （PcG） proteins form multiprotein complexes that regulate

expression patterns of developmental and cell proliferation genes. Several

members of the PcG contain ring finger domains and are identified as a subclass

of RING finger proteins. The RING-type zinc finger motif is present in a

number of viral and eukaryotic proteins and is made of a conserved cysteinerich

domain that is able to bind two zinc atoms. Proteins that contain the

RING-type zinc finger conserved domain are generally involved in the ubiquitination

pathway of protein degradation. PCGF6 （polycomb group ring finger

6）, also known as MBLR or RNF134, is a 350 amino acid nuclear protein

that is ubiquitously expressed and contains one RING-type zinc finger. PCGF6

acts as a transcriptional repressor and regulates the level of histone

H3K4Me3 by activating SmcY histone demethylase. PCGF6 exists as two

alternatively spliced isoforms.

REFERENCES

1. Kanno, M., Hasegawa, M., Ishida, A., Isono, K. and Taniguchi, M. 1995.

Mel-18, a Polycomb group-related mammalian gene, encodes a transcriptional

negative regulator with tumor suppressive activity. EMBO J. 14:

5672-5678.

2. Satijn, D.P., Gunster, M.J., van der Vlag, J., Hamer, K.M., Schul, W.,

Alkema, M.J., Saurin, A.J., Freemont, P.S., van Driel, R. and Otte, A.P.

1997. RING1 is associated with the polycomb group protein complex and

acts as a transcriptional repressor. Mol. Cell. Biol. 17: 4105-4113.

3. Joazeiro, C.A. and Weissman, A.M. 2000. RING finger proteins: mediators

of ubiquitin ligase activity. Cell 102: 549-552.

4. Akasaka, T., Takahashi, N., Suzuki, M., Koseki, H., Bodmer, R. and Koga, H.

2002. MBLR, a new RING finger protein resembling mammalian Polycomb

gene products, is regulated by cell cycle-dependent phosphorylation. Genes

Cells 7: 835-850.

5. Tuckfield, A., Clouston, D.R., Wilanowski, T.M., Zhao, L.L., Cunningham,

J.M. and Jane, S.M. 2002. Binding of the RING polycomb proteins to specific

target genes in complex with the grainyhead-like family of developmental

transcription factors. Mol. Cell. Biol. 22: 1936-1946.

6. Online Mendelian Inheritance in Man, OMIM™. 2003. Johns Hopkins

University, Baltimore, MD. MIM Number: 607816. World Wide Web URL:

http://www.ncbi.nlm.nih.gov/omim/

7. Moore, R. and Boyd, L. 2004. Analysis of RING finger genes required for

embryogenesis in C. elegans. Genesis 38: 1-12.

8. Yang, Y., Lorick, K.L., Jensen, J.P. and Weissman, A.M. 2005. Expression

and evaluation of RING finger proteins. Meth. Enzymol. 398: 103-112.

9. Lee, M.G., Norman, J., Shilatifard, A. and Shiekhattar, R. 2007. Physical and

functional association of a trimethyl H3K4 demethylase and Ring6a/MBLR,

a polycomb-like protein. Cell 128: 877-887.

STORAGE

Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of

shipment. Non-hazardous. No MSDS required.

CHROMOSOMAL LOCATION

Genetic locus: PCGF6 （human） mapping to 10q24.33; Pcgf6 （mouse） mapping

to 19 C3.

SOURCE

PCGF6 （E-15） is an affinity purified goat polyclonal antibody raised against a

peptide mapping within an internal region of PCGF6 of human origin.

PRODUCT

Each vial contains 200 μg IgG in 1.0 ml of PBS with < 0.1% sodium azide

and 0.1% gelatin.

Blocking peptide available for competition studies, sc-160649 P, （100 μg

peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA）.

APPLICATIONS

PCGF6 （E-15） is recommended for detection of PCGF6 of mouse, rat and

human origin by Western Blotting （starting dilution 1:200, dilution range

1:100-1:1000）, immunofluorescence （starting dilution 1:50, dilution range

1:50-1:500） and solid phase ELISA （starting dilution 1:30, dilution range

1:30-1:3000）; non cross-reactive with PCGF1, PCGF3 or PCGF5.

Suitable for use as control antibody for PCGF6 siRNA （h）: sc-90663, PCGF6

siRNA （m）: sc-152109, PCGF6 shRNA Plasmid （h）: sc-90663-SH, PCGF6

shRNA Plasmid （m）: sc-152109-SH, PCGF6 shRNA （h） Lentiviral Particles:

sc-90663-V and PCGF6 shRNA （m） Lentiviral Particles: sc-152109-V.

Molecular Weight of PCGF6: 60 kDa.

RECOMMENDED SECONDARY REAGENTS

To ensure optimal results, the following support （secondary） reagents are

recommended: 1） Western Blotting: use donkey anti-goat IgG-HRP: sc-2020

（dilution range: 1:2000-1:100,000） or Cruz Marker™ compatible donkey

anti-goat IgG-HRP: sc-2033 （dilution range: 1:2000-1:5000）, Cruz Marker™

Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent:

sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2） Immunofluorescence:

use donkey anti-goat IgG-FITC: sc-2024 （dilution range: 1:100-

1:400） or donkey anti-goat IgG-TR: sc-2783 （dilution range: 1:100-1:400）

with UltraCruz™ Mounting Medium: sc-24941.

RESEARCH USE

For research use only, not for use in diagnostic procedures.

PROTOCOLS

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