sigmaDL-二硫苏糖醇DL-Dithiothreitol

CAS number: 3483-12-3

Product Description:

Appearance: White Powder

Molecular formula: C4H10O2S2

Melting Point: 42-43°C

Molecular weight: 154.1 g/mol

Solubility: 0.1 M in H2O, 20°C, complete, colorless

pH: 4.0-6.5 （0.1 M in H2O, 25°C）

This product is designated as BioChemika Ultra for Molecular Biology grade and is suitable for applications in the molecular biology, specially if high purity is required. It has been analyzed for low contaminating impurity and the absence of nucleases.

DTT is synthetic. A method of preparation has been described in J. Chem. Soc. 253 （1949）

DTT is an agent for reducing -S-S- to -SH. Redox potential: -0.33 volts at pH 7. DTT acts as a protective agent for -SH groups of other species in solution. In this reaction the DTT is oxidized to the cyclic disulfide, and thereby ensures the reduction of other disulfides in solution. The disulfide reduction is complete in minutes at pH 8. A specific and sensitive assay for disulfide groups is based on determination of resulting monothiols with DTNB （Fluka 43760）.2

Applications:

It is used in buffers for isolation of RNA5 or buffers used for RT-PCR starting with mRNA6,7.

Precautions and Disclaimer:

For Laboratory Use Only. Not for drug, household or other uses.

Preparation Instructions:

The solubility of DTT has been tested by Fluka at 50 mg/ml in water, resulting in a clear colorless solution. It has been described as freely soluble in water, ethanol, acetone, ethylate, chloroform and ether.

Solutions oxidize relatively slowly in air.3 It is recommended to make solutions fresh daily. Recorded half-life （hours） of DTT solutions at various pH and temperatures are as follows:4

Conditions （all in 0.1 M KPO4 buffer）

Half life （Hours）

pH 6.5 at 20°C

40

pH 7.5 at 20°C

10

pH 8.5 at 20°C

1.4

pH 8.5 at 0°C

11

pH 8.5 at 40°C

0.2

pH 8.5 at 20°C +0.1 mM Cu2+

0.6

pH 8.5 at 20°C +0.1 mM EDTA

4

Storage/Stability:

Storage Temperature: 4°C

When stored as indicated, this product is stable for years.

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